Figure 5.
Test for the toxicity of the Dam-Lamin Dm0 (Dam-LAM) protein for Drosophila. First, flies bearing two transgenes, βTub85D-FLP and P.hsp70FL-STOP#2-Dam-LAM, were obtained. The βTub85D-FLP transgene is expressed during spermatogenesis (44). The STOP#2 cassette contains a functional yellow (y+) gene (45). In the progeny of males carrying the two transgenes, the following three classes were expected: [1] flies without Dam-LAM transgene, [2] flies with the intact (non-recombined) transgene and [3] flies with shortened (FRT-recombined) transgene. Male adults were counted on days 1–3 after eclosion. Experiments were performed at two different temperatures, 18°C and 25°C. w+mC, mini-white gene. P.hsp70FL, full-length hsp70 promoter.