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. 2016 Apr 11;44(12):5892–5907. doi: 10.1093/nar/gkw241

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Translational regulation of EMT-inducing genes is upregulated by TGFβ and knockout of hnRNP E1. (A) Immunoblot analysis of candidate motif containing genes where mRNA was previously identified as showing an inherent interaction with hnRNP E1 and whose polyribosomal induction was mediated by TGFβ stimulation. NMuMG cells and a mutant hnRNP E1 knockout version, NMuMG-/-hnRNP E1 were treated with 5 ng/ml TGFβ up to 24 h. (B) Semi-quantitative PCR analysis of mRNA levels from lysates used in (A). (C) Quantitative real-time PCR analysis of mRNA candidate genes, normalized to expression of GAPDH mRNA levels. Results are depicted as means +/− s.e.m. of three independent biological experiments.