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. 2016 Apr 19;44(12):5924–5935. doi: 10.1093/nar/gkw276

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — pab-1 and pab-2 genetically cooperate with let-7 and lsy-6 miRNAs. (A) pab-1 and pab-2 genetically interact with the let-7 miRNA. let-7(n2853) animals (L1) were fed with bacterially-expressed dsRNA against the indicated gene, or L4 animals injected with dsRNA (B) and F1 animals were scored for bursting vulva phenotype at the permissive temperature (16°C). Results shown are representative of at least two experimental and biological replicates. (C) pab-2 genetically interacts with lsy-6. pab-2(0)(ok1851) were crossed with OH3646 (otIs114(Plim-6::GFP, rol-6(d));lsy-6(ot150). Animals were scored for expression of GFP in the ASEL.