Figure 3.

Fraction of long-lived RPA–DNA complexes. (A) The ‘dwell time’ distribution for RPA binding to dT35, dT25, dT20 and dT15 with a 40-s cutoff indicated (dashed line). (B) Quantification of the fraction of events with dwell-times longer than 40 s for RPA, AroA, AroB and Aro2 with each of the oligonucleotides analyzed. Error bars indicate standard errors from three experiments. No long-dwell complex (>40 s) were detected AroB and Aro2 with dT20 (ND) and no binding events were observed with the Aro mutants with dT15 (NE).