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NusG-NTD interacts with NusA. [1H,15N]-HSQC spectra of (A) 15N-NusG, (B) 15N-NusG-NTD and (C) 15N-NusG-CTD before, black, and after, red, addition of NusA in equimolar concentration. Numbers in (A) represent the corresponding amino acid number of NusG with NusG-NTD signals in red and NusG-CTD signals in black. The concentration of the NusG construct was 50 μM in all experiments.
