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In vitro transcription assay for combinations of NusG, NusA, NusG-NTD and NusA-AR2. 23 % urea-polyacrylamide gel. The assembled TEC (TEC11A) was pre-incubated with NusG, NusA, NusA-AR2, NusG-NTD, combinations of these, or transcription buffer for 10 min at 25°C. Transcription was started by NTP addition and stopped after 60 s. TEC11A, run-off, and three pause sites are indicated by arrows.
