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. 2016 May 24;44(12):5983–5992. doi: 10.1093/nar/gkw465

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Kinetic characterization of selected glycine fluorescence riboswitches on chip. (A) Top: fluorescence images representing the response time of the riboswitch (T10A9). For this the glycine concentration was rapid exchanged over the pulled-down areas on the microfluidic chip and the fluorescence signal change recorded. Bottom: representative fluorescence time traces of two riboswitches upon addition of 0.01 M glycine (left) and complete depletion of glycine (right). B) Apparent k^on and k^off rates for 12 fluorogenic glycine riboswitches with different transmitter-actuator lengths. The rates were obtained by fitting a single exponential function to the fluorescence time traces.