Figure 5.

Temperature dependence of the apparent k^on and k^off rates of glycine fluorescence riboswitches with different transmitter-actuator lengths. (A) Representative fluorescence time traces of the riboswitch T10A9 upon addition of 0.01 M glycine (left) at three different temperatures. The corresponding apparent k^on rates are plotted against the temperature to obtain the apparent energy barrier for the glycine binding reaction. (B) Apparent energy barriers for glycine binding () and dissociation () to the fluorogenic glycine riboswitches with different transmitter-actuator lengths.