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Oligonucleotide sequences and 2′-deoxynucleotides used for template-directed primer extension reaction. Assays at increasing concentrations of activated monomers were performed using the following conditions: 36 μM template: 3′-aminoprimer complex, 0.18 to 7.2 mM matching activated monomer (2a–t or 3a–t) in primer extension buffer (HEPES 200 mM, NaCl 400 mM, MgCl2 80 mM), pH 8.9 (for OAt-dNMP) or 7.0 (for MeIm-dNMP) at 20°C.
