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Oligoribonucleotide sequences and ribonucleotide monomers used for template-directed primer extension assays. Conditions: 36 μM template:primer complex, 1.8 to 60 mM complementary monomer (5a–u or 6a–u) in primer extension buffer (200 mM HEPES, 80 mM MgCl2, 400 mM NaCl, pH 8.9 (for OAt-NMP) or 7.7 (for MeIm-NMP) at 20°C.
