Figure 8.
Ratios of rate constants for the covalent step of extension (kcov) and hydrolysis (khydr) for different nucleotides and OAt or MeIm as leaving group. The larger the value, the more likely that a bound monomer will react with the primer, whereas a low ratio means that the given monomer is more likely to suffer hydrolysis. (A) kcov/khydr ratios for deoxynucleotide monomers reacting with aminoterminal primers, (B) kcov/khydr ratios for those ribonucleotides reacting with RNA primers for which reliable data is available. Note the much smaller absolute values compared to A).