Table 1. Summary of the mitochondrial translation factors studied, including the bacterial homologs and mutant phenotypes.
Mitochondrial translation factors | Bacterial homologs | % identity S. cerevisiae/E. coli | S. cerevisiae mutants used in this study | % rho- 28°C |
---|---|---|---|---|
Ifm1 | IF2 | 33 | Δifm1 | 20 ± 10 |
Mif3/Aim23 | IF3 | <20 | Δmif3 | <5 |
Rrf1 | RRF | 20 | Δrrf1 | <5 |
Mrf1 | RF1 | 35 | Δmrf1 | 100 |
mrf1 (R231G) | 20 ± 10 | |||
Mef2 | EF-G | 29 | Δmef2 | 100 |
mef2 (K54A) | 10 ± 5 | |||
Nam2 | LeuRS | 32 | Δnam2 | 100 |
nam2 (W238C) | <5 |