Figure 9.
CrbCmlA-mediated translational pausing. (A) Map of construct for quantifying pausing. The construct consists of (from left to right) the lac promoter, N-terminal end of the lacZ gene (gray), a piece of pGEM5 (white box), the sequence for the crb pausing peptide, intergenic hairpin and the N-terminus of cmlA (all black) and finally the remaining part of the lacZ gene (gray). For more details, see ‘Materials and Methods’ section. (B) β-galactosidase enzyme activity. Enzyme activity in the absence (white bars) or presence of 0.8 μg/ml chloramphenicol (CM) (black bars) are shown. The averages and standard errors of the mean (gray vertical lines) of biological triplicates are shown. Three enzyme assays were done for each replicate.