Figure 4.

hPMR1 downregulates pre-miR-200a and 200c. (A) Cytoplasmic RNA from untreated and doxycycline-treated cells carrying the active hPMR1 transgene was analyzed by RT-qPCR for pre-miR-26a, pre-miR-200a and pre-miR-200c. (B) The experiment in A was repeated using cytoplasmic RNA from cells carrying the catalytically-inactive hPMR1 transgene. (C) RT-qPCR for pre-miR-26a, pre-miR-200a and pre-miR-200c was performed using cytoplasmic RNA from cells that were transduced with lentivirus expressing control (Ctrl) shRNA or shRNA targeting hPMR1. In each case the results represent the mean ± standard deviation (n = 3), and P values by Student's t-test for each determination having statistically significant differences are shown above the respective datasets.