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. 2016 Jul 8;6:29275. doi: 10.1038/srep29275

Table 1. Plasmids used in this study.

Plasmid name Promoter/gene fusion Description Source or Reference
pACYC184   origin p15A (15 copies per cell), CmR, TetracyclineR New England Biolabs
pACYC184ts   origin p15A (15 copies per cell), CmR, with a deletion in the tetracycline resistance gene 37
phfq   pACYC184-derivative carrying Y. enterocolitica hfq 37
pYopH-L-Bla   pACYC184 derivative carrying the promoter and coding sequence of the yopH gene (without stop codon) fused in frame through linker (L) peptide (GGGGS)3 to TEM-1 β-lactamase gene bla, CmR This study
pFX-P   origin RSF1010 (10-12 copies per cell), SpR, gfp gene without start codon, used to generate translational fusions 43
pFX-0 Promoter-less /gfp pFX-P carrying a promoter-less complete gfp gene, used as a negative control, SpR 43
pFX-1 Plac/gfp pFX-P carrying a complete gfp gene under control of the Plac promoter 43
pFX-2 PLtetO-1/gfp pFX-P carrying a complete gfp gene under control of the PLtetO-1 promoter This study
pFX-ail Pail /ail‘-’gfp pFX-P carrying 380 bp upstream of ail start codon, and 9 codons of ail fused in frame with gfp This study
pFX-PtetO-ail PLtetO-1/ail‘-’gfp pFX-P carrying the PLtetO-1 promoter, 59 bp upstream of ail start codon, and 9 codons of ail fused in frame with gfp This study
pFX-ompX PompX/ompX‘-’gfp pFX-P carrying 402 bp upstream of ompX start codon, and 5 codons of ompX fused in frame with gfp This study
pFX-Plac-ompX Plac/ompX‘-’gfp pFX-P carrying the Plac promoter, 44 bp upstream of ompX start codon, and 5 codons of ompX fused in frame with gfp This study
pFX-invA PinvA/invA‘-’gfp pFX-P carrying 494 bp upstream of invA start codon, and 4 codons of invA fused in frame with gfp This study
pFX-Plac-invA Plac /invA‘-’gfp pFX-P carrying the Plac promoter, 121 bp upstream of invA start codon, and 4 codons of invA fused in frame with gfp This study
pFX-yadA PyadA/yadA‘-’gfp pFX-P carrying 686 bp upstream of yadA start codon, and 16 codons of yadA fused in frame with gfp 22
pFX-Plac-yadA Plac/yadA‘-’gfp pFX-P carrying the Plac promoter, 264 bp upstream of yadA start codon, and 16 codons of yadA fused in frame with gfp This study
pFX-rovA ProvA/rovA‘-’gfp pFX-P carrying 634 bp upstream of rovA start codon, and 6 codons of rovA fused in frame with gfp This study
pFX-ompR PompR/ompR‘-’gfp pFX-P carrying 188 nucleotides upstream of ompR start codon, and 4 codons of ompR fused in frame with gfp This study
pFX-phoP PphoP/phoP‘-’gfp pFX-P carrying 860 bp upstream of phoP start codon, and 5 codons of phoP fused in frame with gfp This study
pFX-myfA PmyfA/myfA‘-’gfp pFX-P carrying 173 bp upstream of myfA start codon, and 7 codons of myfA fused in frame with gfp This study
pFX-plac-myfA Plac/myfA‘-’gfp pFX-P carrying the Plac promoter, 99 bp upstream of myfA start codon, and 7 codons of myfA fused in frame with gfp This study