Figure 8. The in vivo and ex-vivo typical fluorescence images.

The mice bearing 4T1 tumors were injected one time with DIR labeled MTX injection and nanoparticles (MTX equivalent, 8 mg Kg⁻¹): In vivo images of mice after 0.5, 1, 3, 6, 9, 12, and 24 h post injection (a), ex-vivo fluorescence images of main tissues, all the organs and tumors were placed in the order of (heart, liver, spleen, lung, kidney, brain, and tumor) after 24 h post injection (b), intensity of fluorescent signal in organs and tumor after 24 h post injection (c). ^*p < 0.01 vs. MTX injection.