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. 2016 Jul 8;6:29256. doi: 10.1038/srep29256

Figure 1. Proteolytic cleavage of EGFR by CTSS.

Figure 1

(a) Recombinant EGFR was incubated with CTSS (left panel) and CTSB (right panel) at 37 °C for the indicated durations. The reaction was stopped by adding a sample buffer, and the reaction mixtures were subjected to SDS-PAGE, followed by Western blotting with an EGFR antibody. (b) Recombinant EGFR was incubated with CTSS in the presence of a CTSS inhibitor 6r or ZFL. The reaction mixtures were incubated at 37 °C for 20 min and then subjected to SDS-PAGE.