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. 2016 Jul 8;6:29417. doi: 10.1038/srep29417

Figure 3. HO-1 inhibition impairs directional migration of EC.

Figure 3

(A) HUVEC were transfected with control siRNA (CTL), HO-1 siRNA (seq2) or pooled HO-1 siRNAs (pool). Cells were seeded onto growth factor-reduced Matrigel in the presence or absence of VEGF for 16 h. Images from five fields per view were captured, and (B) tube length was quantified using ImageJ software (NeuronJ). (C) Murine EC from Hmox1−/− mice or Hmox1+/+ littermate controls were seeded onto growth factor-reduced Matrigel in the presence or absence (UT) of VEGF for 16 h. Images from five fields per view were captured and (D) tube length quantified using ImageJ. Pictures are representative of 3 experiments (Bars = 50 μm). Data are presented as mean ± SEM (n = 3 experiments), *p < 0.05, **p < 0.01, ***p < 0.001.