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. 2016 Jul 8;6:29417. doi: 10.1038/srep29417

Figure 4. HO-1-deficiency results in endothelial cell cycle arrest.

Figure 4

(A) Control siRNA (siCTL)-transfected or pooled siHO-1-transfected HUVEC were left untreated or treated with 25 ng/ml VEGF for 48 h prior to propidium iodide staining and flow-cytometric analysis of DNA distribution. Data is presented as mean ± SEM (n = 3 experiments), *p < 0.05 vs siCTL, δp < 0.05 vs siCTL + VEGF. (B) HUVEC were exposed to geneFECTOR alone (GF) or transfected with control siRNA (CTL) or pooled HO-1 siRNA (HO-1). Cells were cultured in the presence or absence of VEGF for 24 h prior to mRNA quantification by qRT-PCR of (B) cyclin A1, (C) cyclin E1, (D) cyclin-dependent kinase 2 (cdk2) or (E) p27. Data are presented as mean ± SEM (n = 3 experiments), *p < 0.05, **p < 0.01. δδp < 0.01 vs untreated siCTL-transfected cells.