Figure 5. Cyclin A1 is reduced in migrating HO-1-deficient EC.

HUVEC were transfected with CTLAF₄₈₈ or HO-1 siRNA (seq2AF₄₈₈). Monolayers were scratched and treated with vehicle alone or VEGF (25 ng/ml) for 16 h (A) Migration was quantified by live cell imaging using ImageJ. EC were then harvested, lysed and immunoblotted for: (B) cyclin A1 and (C) retinoblastoma protein (pRb) and phospho-pRb^pT821. The histograms show corresponding densitometry data corrected for the GAPDH loading control and with phospho-pRb expressed relative to total pRb. Data are presented as mean ± SEM (n = 3 experiments), *p < 0.05, **p < 0.01; ***p < 0.001.