Table 2. Mankin scoring of DMM joint histology by individual component.

Parameter	WT			cKO
	Control	DMM	Limb(p-value)	Control	DMM	Limb(p-value)
Cartilage Degeneration	9.9 ± 1.1	18.6 ± 2.6*	0.007	7.5 ± 0.8	13.8 ± 2.3*	0.0419
Safranin-O Loss	12.4 ± 1.2	19.5 ± 2.2*	0.0113	10.2 ± 0.1	15.8 ± 1.9*	0.0083
Tidemark Duplication	0.24 ± 0.12	0.87 ± 0.41	0.375	0.071 ± 0.71	0.071 ± 0.71	1
Chondrocyte cloning	0.31 ± .08	0.26 ± 0.09	0.8438	0.38 ± 0.12	0.33 ± 0.10	0.7646
Hypertrophic Chondrocytes	1.75 ± 0.45	1.80 ± 0.56	0.6975	1.97 ± 0.47	2.07 ± 0.48	0.751
Fibrocartilage	0	0	–	0	0.07 ± 0.05	0.5
Subchondral Bone Thickness	3.91 ± 0.46	3.38 ± 0.43	0.4801	2.62 ± 0.32	3.55 ± 0.42	0.124

Statistical differences between Control and DMM limbs were determined by a matched pairs t test for normally distributed data (Safranin-O loss) or Wilcoxon Sign Rank test for non-normally distributed data (cartilage degeneration, tidemark duplication, chondrocyte cloning, hypertrophic chondrocytes, fibrocartilage, and subchondral bone thickness). No genotype effects were found. Mean ± SEM. *p < 0.05.