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. 2016 Jul 8;6:29499. doi: 10.1038/srep29499

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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This analysis was done by measuring three independent sets of experiments with 5 substrate concentrations (128 μM, 150 μM, 190 μM, 333 μM, 512 μM). The plots are generated by plotting 1/V as a function 1/[S]. The intercept on the vertical axis is 1/V_max, and the intercept on the horizontal axis is −1/K_M.