Figure 2. Dietary effects on intestinal PFKFB3/iPFK2 expression.

Small intestine and primary IECs were isolated following the feeding period. (A) Intestine extracts were examined for iPFK2 amount and JNK signaling using Western blot analysis. (B) IEC expression of PFKFB3 mRNAs was determined using real-time PCR. (C) Western blot analysis of IEC iPFK2 amount. (D) Quantification of IEC iPFK2 amount. For bar graphs, data are means ± SEM, n = 4–6. HFD, high-fat diet; LFD, low-fat diet; JNK, c-Jun n-terminal kinase.