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. 2016 Jul 8;6:28963. doi: 10.1038/srep28963

Figure 3. Dietary effects on IEC proinflammatory responses.

Figure 3

Primary IECs were isolated following the feeding period. (A) Western blot analysis of IEC JNK signaling. (B) Quantification of IEC Pp46/p46. (C) IEC expression of IL-6, TNFα, and TLR4 mRNAs. For (C) data are means ± SEM, n = 4–6. *P < 0.05; **P < 0.01 HFD vs. LFD for the same gene. HFD, high-fat diet; IL-6, interleukin-6; iPFK2, inducible 6-phosphofructo-2-kinase; LFD, low-fat diet; Pp46, phosphorylated c-Jun n-terminal kinase 1 (JNK1); p46, total JNK1; TLR4, Toll-like receptor 4; TNFα, tumor necrosis factor alpha.