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. 2016 Jul 8;6:29459. doi: 10.1038/srep29459

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(A) TGX (Tris-Glycine eXtended) stain free gel showing purified (detergent solubilized) proteins and nanodisc (ND) reconstituted proteins (visualized by ImageLab software). (B) Radiolabeled activity assays of purified and ND reconstituted CYP79A1 and (Lane 1 and 2) where tyrosine is added as substrate and the enzymatically produced oxime is detected. For purified and reconstituted CYP71E1 (Lane 3 and 4), the substrate oxime is converted to aldehyde. p- Hydroxyphenylacetonitrile, a known biosynthetic intermediate produced by CYP71E1 is observed above the position of the oxime. (C) Cytochrome c assays for purified (detergent solubilized POR) and POR ND. The turnover rate was measured by determining the slope in the linear region of the curve. (The peak at 0.1 min is due to addition of NADPH for reaction initiation).