Figure 4. The importance of membrane ERα signaling is tissue-dependent.

12-week-old NOER and wild type (WT) mice were ovariectomized (ovx) and treated with 17β-estradiol (E2, 16,7 ng·mouse⁻¹·day⁻¹) or placebo (P) for four weeks. Estrogen effects on organ weights are given as weight per body weight (BW). Uterus weight (a), thymus weight (b), % total body fat measured by DXA (c), retroperitoneal fat weight (d), gonadal fat weight (e), serum leptin levels (f) and liver weight (g). Values are given as mean ± sem. [n = 10–12]. *p < 0.05, **p < 0.01, ***p < 0.001, student’s t-test, E2 vs placebo treatment. ^###p < 0.001, interaction P value from two-way-ANOVA analysis, E2 effect in NOER vs E2 effect in WT.