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Phenotype of Ingra and Ingra/Cpslo17 F1. Pollen I2-KI staining of Ingra (A) and F1 (B). Spikelet of Ingra (left) and F1 (right), the arrow indicates the shriveled grain (C). In vitro pollen germination (D and E), germination of pollen on stigma (F and G), and pollen tube elongation in ovary (H and I) showed no difference between Ingra (D, F, and H) and F1 (E, G, and I). Bars: (A, B, D, and E) 50 μm; (C) 1 cm; and (F–I) 500 μm.
