Table 2. AVG cross-over defects are rescued by an aex-3-containing fosmid, expression of the cDNA in AVG and unc-5 overexpression (% animals with defects).
| Genotype | AVG CO | n |
|---|---|---|
| aex-3 fosmid (WRM0629Da08) | ||
| aex-3(hd148); nid-1; hdEx602(+) | 24** | 102 |
| aex-3(hd148); nid-1; hdEx602(−) | 50 ns | 104 |
| aex-3 cDNA | ||
| aex-3(hd148); nid-1; hdEx610(+) | 18** | 128 |
| aex-3(hd148); nid-1; hdEx610(−) | 45 ns | 95 |
| aex-3(hd148); nid-1; hdEx611(+) | 16** | 130 |
| aex-3(hd148); nid-1; hdEx611(−) | 48 ns | 100 |
| aex-3(hd148);nid-1; hdEx612(+) | 13** | 116 |
| aex-3(hd148); nid-1; hdEx612(−) | 47 ns | 94 |
| unc-5 overexpression | ||
| aex-3(hd148); nid-1; evIs98c | 25** | 132 |
Marker used: hdIs51[odr-2::tdTomato]. n = number of animals. For rescue experiments, animals with (+) and without (−) extrachromosomal arrays were counted. For the aex-3 rescue the following independent transgenic lines were analyzed: hdEx602 [WRM0629dA08; myo-2::GFP]; hdEx610, 611, and 612 [plin-11::aex-3 cDNA::GFP; unc-122]; and evIs98c [unc-5p::UNC-5::GFP]. For statistical significance, we compared transgenic lines with aex-3(hd148); nid-1 double mutant. ** P < 0.01; ns, not significant; χ2 test.