Table 3. Other neuronal defects in nid-1 single and aex-3(hd148); nid-1 double mutants (% animals with defects).
| Phenotype | aex-3(hd148) | nid-1(cg119) | aex-3(hd148); nid-1 |
|---|---|---|---|
| Interneuron cross-over defects | |||
| AVG dependenta | 6 (101) | 6 (114) | 35** (104) |
| AVG independentb | 5 (101) | 0 (114) | 14** (104) |
| AVK defects | |||
| VNC cross-over | 6 (107) | 18 (115) | 49** (109) |
| Premature stop | 0 (107) | 12 (115) | 22 ns (109) |
| Leaving VNC | 0 (107) | 1 (115) | 3 ns (109) |
| HSN neurons | |||
| VNC cross-over | 9 (132) | 58 (103) | 68 ns (109) |
| Undermigration and VNC cross-over | 6 (132) | 3 (103) | 16** (109) |
| Motor neuron defects | |||
| DD/VD | |||
| VNC cross-overc | 6 (102) | 8 (133) | 28** (123) |
| Symmetrical VNC | 0 (102) | 0 (133) | 36** (123) |
| Commissural defects | 14 (102) | 65 (133)d | 100** (123)e |
| DNC gaps | 31 (95) | 85 (53) | 100* (26) |
| DA/DB | |||
| VNC cross-overf | 6 (101) | 8 (110) | 29** (103) |
| Symmetrical VNC | 0 (101) | 0 (110) | 20** (103) |
| Commissural defects | 0 (101) | 0 (110) | 18** (103) |
Values in parentheses indicate n. Markers used: Interneuron (glr-1::GFP), AVK (flp-1::GFP), HSN (tph-1::GFP), DD/VD/Commissures (unc-47::DsRed-2), and DA/DB (unc-129::CFP). For statistical significance, aex-3; nid-1 double mutants are compared with nid-1 single mutant. * P < 0.05; ** P < 0.01; ns, not significant; χ2 test.
a
Interneurons cross the midline together with AVG.
b
Interneurons cross the midline without AVG.
c
DD/VD axons cross the midline at the same position where the AVG axon crosses.
d
On average, 5 of 19 commissures fail to reach the DNC per animal.
e
On average, 7 of 19 commissures fail to reach the DNC per animal.
f
DA/DB axons cross the midline at the same position where the AVG axon crosses.