Comparison of two gynogenetic families of sex-reversed physiological females ♀(+)I and ♀(+)II. (A) FISH analysis in metaphases of sex-reversed physiological females ♀(+)I (left panel) and ♀(+)II (right panel). (B) Histogram analysis of microchromosome number in ♀(+)I (left panel) and ♀(+)II (right panel). The number of microchromosomes is indicated on the x-axis, and the percentage of total cells is shown on the y-axis. (C) Sex ratios in the gynogenetic families of ♀(+)I (left panel) and ♀(+)II (right panel). (D) Detection of the male-specific SCAR marker in 20 randomly-picked females, 20 randomly-picked males, and parental individuals in each family. ♀, female; ♂, male; (+), with male-specific marker; (-), without male-specific marker; Cc, C. carpio.