Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 8;6:28891. doi: 10.1038/srep28891

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Scheme of workflow. Cells were first selected for the M2 transactivator and MITF reporter and afterwards transduced with transcription factor cocktail and induced with doxycycline (B) Transgene expression was confirmed with different transcription factor combinations. Stronger RFP signal was observed depending on the amount of different factors used. Various transcription factor cocktails could induce MITF expression in MET-4 cells. The strongest expression was noted for the factor combination MITF, LEF-1, SOX9 and SOX10. (C) Different transcription factor combinations could induce morphological changes in MET-4 cells. The cells changed their morphology from cobblestone-like appearance into more spindle-shaped cells. Cell-cell adhesion was lost and colony formation was reduced. Single cells with loose contacts to the neighbouring cells were present. (D) Transgene expression (RFP) and MITF reporter activation (GFP) in HaCaT and MET-4 cells for different factor combinations (E) Morphology of HaCaT cells, MET-4 cells and melanocytes as well as morphological changes observed after induction of the transdifferentiation factor overexpression in melanocyte-transdifferentiated HaCaT (MT-HaCaT) and melanocyte-transdifferentiated MET-4 (MT-MET-4) cells.