Fig. 3.

Evaluation of enrichment methods for glycosylated peptides and proteins according to the type of glycosylation and cell conditions. Glycosylation was compared between proteins from crude samples, proteins and peptides eluted from ConA columns and crude samples digested by Endo H, according to the nature of the glycosylation (N- or O-glycosylations on Asn and Ser or Thr residues, respectively) and the cell conditions (WT, WT-F, mca1Δ/Δ and mca1Δ/Δ-F). Glycosylated peptides were identified with the Byonic search engine.