Table III. Examples of candidate substrates identified by the analysis of proteins eluted from ConA columns, digested with the GluC enzyme and validated by the label-free approach. Peptides were considered to be putative substrates if they were identified in at least two of the three replicates in WT-F conditions only and had a K or R amino-acid residue at one end. Putative substrates were validated by a label-free approach and those with an abundance higher in WT-F conditions than in other conditions (with a ratio greater than 2) and an ANOVA p value < 0.05 were considered to be highly probable substrates.

Accession	Description	Sequences	Log Prob Byonic	Identifications (3 replicates) Byonic scores	Label-free quantitation
					Ratio WT-F/WT	Ratio WT-F/mca1	Ratio WT-F/mca1-F	Anova
orf19.6190	SRB1; Essential GDP-mannose pyrophosphorylase; makes GDP-mannose for protein glycosylation	MKGLILVGGYGTR	4.42	116.2, 232.4	infinity	infinity	26.4	0.0014
orf19.4565	BGL2; Cell wall 1,3-beta-glucosyltransferase; mutant has cell-wall and growth defects, but wild-type 1,3- or 1,6-beta-glucan content	GDLDFLKSHSK	9.27	480.5, 391.7, 532.1	96.6	900.2	11.9	0.0009
		LASKINDIKDLVK	8.2	497.5, 257.9, 547.9	96.5	29	6.1	0.015
orf19.1738	UGP1; UTP-glucose-1-phosphaturidyl transferase; localizes to yeast, not hyphal cell surface; Hog1-repressed	HGALVLDPTRDGFSNPLIKLGSHFK	10.07	447.9, 340.3, 546.1	277.5	140	16.7	0.008
orf19.3010.1	ECM33; GPI-anchored cell wall protein; mutants show cell-wall defects and reduced adhesion, host cell damage, and endocytosis	RVSGGFILKTD	3.36	321, 219.4, 229.8	Infinity	Infinity	18	1.1E-08
		LTGVQQIYGSLHVK	7.83	313.1, 435, 363.2	31.1	21.8	13.9	0.0002
orf19.744	GDB1; Putative glycogen debranching enzyme	KALGAIIIADK	7.04	399.5, 403.4	22.3	46.3	5.3	6.9E-5
orf19.338	Uncharacterized ORF; Putative glycoside hydrolase; stationary phase enriched protein; Hog1p-downregulated	YFDGDSGRGLGASHQTGWTSLVTK	6.49	39.1.3, 356.9, 375.1	103.6	28.6	5.4	1.42E-6
orf19.2340	CDC48; Putative microsomal ATPase; plasma membrane-localized	LVELPLRHPQLFK	8.06	470.3, 458.1, 473.4	Infinity	237.5	15.1	0.0003
orf19.6367	SSB1; HSP70 family heat shock protein	VVLVGGSTRIPKVQKLLSDFFDGK	3.52	120.8, 174.3, 87.3	316.5	33.4	8.1	0.003