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Epitope mapping for MBP. Eight C-terminal deletion fragments were constructed for each isoform of MBP. Two response patterns were observed. In pattern 1, reactivity to deletion mutants MBP 1.7, MBP 1.8, MBP 2.2 and MBP 2.3 were observed. In pattern 2, reactivity to deletion mutants MBP 1.8 and MBP 2.3 were observed, but not to MBP 1.7 and MBP 2.2. MBP 1.7 and MBP 2.2 shared the same sequence and MBP 1.8 and MBP 2.3 shared same sequence.
