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. 2016 Jul;15(7):2379–2395. doi: 10.1074/mcp.M116.058115

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — CAX-PAGE differential separation of sham and SCI rat lysate (A) Ion exchange (CAX) separation chromatograms overlay of pooled rat SCI (at 24 h and 7 day postsevere injury) and sham at 24 h and 7 day lysate (pooled from n = 5 each) with the same 280 nm absorbance scale: sham in purple and SCI in green, solvent B % in orange, and conductivity in red. B, 1D-PAGE of selected CAX fractions out of 28 collected, run side by side for comparison, selected bands are boxed and labeled according to 2D position. C, Quantification of selected differential gel band intensities to derive the relative -fold increase or decrease using ImageJ densitometry software.