Effects with the removal of iAs-treatment after transformation (iAs-rev cells).
A, Western blot analyses of EMT markers. Claudin-3 and Slug in iAs-rev cells show an intermediate level of expression, whereas others such as β-Catenin and Vimentin revert to near NT levels. B, qRT-PCR for EMT markers. qRT-PCR for EMT markers in iAs-Rev cells mostly show changes in expression opposite of the iAs-T cells. C, qRT-PCR of the H2B variants. qRT-PCR for H2B variants in iAs-Rev cells mostly show changes in expression reverting to near NT cells. Error bars represent S.D. from three biological replicates, each containing three technical replicates. *, p < 0.05 compared with NT; †, p < 0.05 between iAs-T and iAs-Rev.