Table II. Proteins and protein complexes identified using top-down proteomics in native mode (nTD). Protein accession numbers in UniprotKB (Accession #), protein-protein interaction (Complex), average theoretical mass, average observed mass of monomers and complexes masses in parentheses, and post-translational modifications (PTM) are included.
Protein | Accession # | Complex | Theoretical mass | Observed mass | PTM |
---|---|---|---|---|---|
Cobra venom factor | I2C090 | – | 140,957 Da | 146,565 +/− 63 Daa | Glycosylation 5,608 Da |
l-amino acid oxidase form 1 | P81383 | Homodimer | 53,691 Da (107,382 Da) | 130,014.2 +/− 0.7 Dab | Glycosylation 22,637.2 Da |
l-amino acid oxidase form 2 | P81383 | Homodimer | 53,690 Da (107,382 Da) | 126,271.0 +/− 2.3 Dab | Glycosylation 18,889.2 Da |
Cysteine rich secretory protein | Q7ZT98 | Homodimer | 24,960 Da (49,920 Da) | 49,918 Da | – |
Phospholipase A2 | Q9DF33 | Homodimer | 13,176 Da (26,352 Da) | 26,375 Da | – |
Snake venom metalloproteinase | D3TTC2 | – | 47,684 Da | 49,283 Dab | Glycosylation 1,599 Da |
a Centered on the top of the glycoform distribution.
b The most abundant glycoform in the distribution.