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Specificity profiling of proteases of the A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family. The protease:library ratio was 1:50 (wt/wt). Incubation at pH 7.5 occurred for 16 h at 37 °C. P1 constitutes the major specificity determinant with a canonical preference for glutamate.
