Fig. 2.

Effect of candidate genes on flowering time in a Dent–Flint introgression library. Adjusted means of (a) male and (b) female flowering times for 97 introgression lines (circles) and the Dent and the Flint parental line (red and blue squares, respectively). Lines carrying a segment with Dent or Flint flowering time candidate genes are highlighted in red or blue, respectively, and lines with a Dent and a Flint candidate are shown in black. The dotted and dashed lines represent the significance thresholds without (α = 0.05) and with correction for multiple testing (α = 0.05/97). Boxplots of adjusted means of flowering times are depicted in the lower parts of (a) and (b) for seven lines carrying Flint haplotypes of Flint flowering time candidates (blue), nine lines carrying Flint haplotypes of Dent flowering time candidates (red), and the 75 lines not carrying a flowering time candidate (gray). For details about the respective lines see Additional file 5: Table S6. Boxplots show the upper and lower quartile, median (horizontal bar), and whiskers (vertical bars) of the adjusted means. Points above and below the whiskers indicate values ± 1.5 times the interquartile range. Significance of Student’s t-tests with p < 0.05 is indicated by *