Figure 4.

RBP expression in Drosophila development. (A) Clustering of z-score-transformed gene expression levels of protein-coding genes expressed during embryogenesis (0–24-h post egg laying) (n = 11,232) (Graveley et al. 2011) into six condensed, timely resolved clusters. (Left) Cluster median z-score-transformed expression values over time. Clusters sizes are indicated in order 1 through 6. (Right) Cluster proportions of all known RBP (harboring RBDs or RNA-binding GO annotation), inferred RBPs (as described in Fig. 2D), and known TFs (FlyTF.org). (B) Median of absolute expression values (log₂[FPKM + 1]) and relative expression values (z-score) of the early embryo mRBPome without ribosomal proteins, known RBPs without ribosomal proteins, and known TFs over time during embryogenesis. (C) Developmental Stage Specificity Score (DSSS) for genes expressed during 30 developmental stages (Graveley et al. 2011) for all protein-coding genes (n=13,452). Scatterplot of DSSS relative to log₂-transformed maximal expression levels for the indicated subsets (left) and heat map of log₂-transformed FPKM + 1 expression values of the 15 most specific members of each subset.