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. 2016 Jun 1;6(6):1177–1230.

Figure 2.

Figure 2

Comparison of the growth of the ALL3 cells at different starting cell densities upon growth factor stimulation. 7 GFs= (KL+FLT3+TPO+IL-3+IL-6+G-CSF+GM-CSF). Unless otherwise stated the GFs concentrations in all experiments were: KL, FLT3, TPO each 50 ng/ml; G-CSF, GM-CSF, IL-3, IL-6, IL-2, hTGF-β, IL-21, IL-1, IL-7, IL-4, IGF-I, IGF-II each 10 ng/ml; and EPO at 1 IU/ml. (KL+FL+TPO@50 ng/ml+G-CSF+GM-CSF+IL-S+IL-6@10 ng/ml) Leptin (100 ng/ml), Note: cell number (y-axis) refers to viable number of cells/ml unless otherwise stated. A, B. Growth of ALL3 cells in liquid culture at starting cell concentrations of 50,000 and 25,000 cells/ml with and without 7 GFs. C. Effect of IGF, FGF, BLys, TGF-β, and all 4 on the growh of ALL3 cells in liquid culture at starting cell concentration of 250,000 cells/ml. D-K. Lack of stimulatory activity of various GFs as shown in legends on the growth of ALL3 cells at 5 × 103, 104, 2 × 104, and 5 × 104 cells/ml.