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. 2016 Jun 1;6(6):1177–1230.

Figure 3.

Figure 3

Single cell cloning of the ALL3 cells and other leukemic cell lines using 60-well single cell cloning plates. A. Cloning of ~10 (2 to 20) ALL3 cells in ALL3 media in 60-well single cell cloning plates. No wells with 1 cell (not shown) or 2-6 ALL 3 cells grew to >100 cell/well (Solid bars) and continued growing, and only a few wells starting with 7-20 cells did so. Some wells started with 13-20 cells grew to >90-125 cells, but then the cells died. The maximum cell count per well at 3-7 days is shown. B. Cloning of 1 to 4 ALL3 cells in ALL3 media in 60-well single cell cloning plates. C. Cloning of single or a few R10- cells in 60 well single cell cloning plates. Only 10% of single cells and 20-27% of 2-5 cells continued growing to >100 cells at 10 days. D, E. Cloning of single or a few K562 cells in 60-well plates. 67% of single and 83% of two RW-Leu4 cells and >50% of single and 94% of two K562 cells per well grew to >100 cells and continued growing as did 96% of the cells in wells containing 3 or more cells of either cell line. Cell counts greater than 100 cells per well are inaccurate because of crowding.