Figure 8.

Effect of supernates from established leukemic cell lines on the growth of the LD ALL3 using [³H]-thymidine uptake assay. A. Growth of the established leukemic cell lines started at HD of 3.5 × 10⁵ cells/ml. B. The viability of all the HD cell lines was 90% or more at both 1 and 3 days determined using the trypan blue exclusion method. C. The effect of supernates collected from the established leukemic cell lines started at HD of 3.5 × 10⁵ cells/ml on the LD ALL3 cells using [³H]-thymidine uptake assay. As expected HD ALL3 SNs were the most stimulatory for the first 11 days while the unstimulated LD ALL3 cells didn’t grow at all. The next most stimulatory was R10- cells which peaked later at day 16. The recorded fold increases of the stimulated over the unstimulated ALL3 cells measures the difference on each day so 1958 on day 16 is not necessarily more stimulatory than 565 on day 11 as more of the control unstimulated LD ALL3 cells have died so their CPM are lower on day 16 than day 11. The SN of HD R10+ and BM185 cells were the next most stimulatory but this occurred still later (Days 16-18). SN of RWLeu4, SKL7, and K562 were not stimulatory at all. The y-axis represents radioactive CPM.