Table 1.
Kinetic profiles (Vmax and Km) of human pancreatic cancer cell lines, murine (PanO2) and Chinese hamster ovary (CHO) cells
| Cell line | Vmax (3H Spd) (nmoles/mg protein/min) | Km for Spd (μM) |
|---|---|---|
| HPNE | 1.8 | 0.19 ± .02 |
| L3.6pl | 24 | 0.67 ± .07 |
| Panc-1 | 13 | 0.31 ± .01 |
| Su86.86 | 7.0 | 0.29 ± .01 |
| BxPC-3 | 9.0 | 0.18 ± .01 |
| AsPC-1 | 2.3 | 0.12 ± .03 |
| Capan-1 | 0.8 | 0.18 ± .02 |
| PanO2 | 9.0 | 0.36 ± .05 |
| CHO | 3.0 | 0.24 ± .06 |
| CHOMG | 0 | ND |
Legend: Aminoguanidine (AG, 250 µM) was added to all pancreatic and HPNE cell lines and AG (at 1 mM) was added for CHO cell experiments to prevent amine oxidase activity present in the fetal bovine serum used for cell culture. These AG concentrations were non-toxic to the respective cell lines in separate experiments (not shown). The Ki values for the PTIs, trimer44 (5a), trimer44NMe (5b) and Lys-Spm (6) in L3.6pl human pancreatic cancer cells, were 36, 55, and 26 nM, respectively.