Table 2.
Cell line sensitivity to DFMO (72 h IC50) and spermidine rescue challengea
| Cell line | 72 h DFMO IC50 (mM) | % viability after 72 h incubation with Spd (1 µM) and the 72 h DFMO IC50 dose |
|---|---|---|
| HPNE | 17 ± 0.6 | 60-62 (Very low) |
| L3.6pl | 4.2 ± 0.11 | 89-93 (High) |
| Panc-1 | 3.8 ± 0.1 | 80-81 (Med) |
| Su 86.86 | 10.4 ± 0.44 | 80-85 (Med) |
| BxPC-3 | 14.4 ± 0.25 | 70-72 (Low) |
| AsPC-1 | 11.8 ± 0.44 | 50-60 (Not rescuable) |
| Capan-1 | 13.2 ± 0.5 | 26-43 (Not rescuable) |
| PanO2 | 0.5 ± 0.03 | 85-93 (High) |
| CHO-K1 | 4.2 ± 0.37 | 100-105 (High) |
| CHOMG | 0.05 ± 0.005 | 50-55 (Not rescuable) |
a
‘Spermidine Rescue Rank’ as measured via the % viability observed when each cell line was incubated 72 h at 37°C in the presence of an IC50 dose of DFMO (listed above) and Spd (1 µM) was assigned as: High (≥ 90%), Medium (≥ 80-89%), Low (≥ 70-79%), Very low (> 60-69%) and not rescuable (≤ 60%).
The rank is shown in parentheses (e.g., High, Med, Low).