Table 4.
Relative Protein expression of Cav-1, ATP13A3 and c-Myc in untreated human pancreatic cancer cell lines along with the respective Vmax values for 3H Spd import and relative Spd rescue indexa
| Cell line | Cav-1 | ATP13A3 | c-Myc | Vmax of untreated cells | Spd rescue index |
|---|---|---|---|---|---|
| HPNE | 98.8 | 13.45 | 1 | 1.8 | Very low |
| L3.6pl | 100 | 100 | 100 | 24 | High |
| Panc-1 | 126.4 | 5.7 | 75.3 | 13 | Med |
| SU86.86 | 118.3 | 9.5 | 30.2 | 7 | Med |
| BxPC-3 | 358.5 | 26.9 | 18.1 | 9 | Low |
| AspC-1 | 16.6 | 1.8 | 4.6 | 2.3 | Not rescuable |
| Capan-1 | 3.3 | 0.0 | 9.0 | 0.8 | Not rescuable |
a
The blots were quantified by Image J software (NIH) and normalized by dividing by the β-actin expression level for each cell line.
Entries represent protein expression and expressed in relative %, with the L3.6pl cell expression levels set to 100%. To illustrate how the relative protein expression patterns relate to the polyamine transport properties of the cell lines, the Vmax value for 3H-Spd import (nmoles Spd/mg protein/min) and the Spd rescue index observed with DFMO-treated cells are listed again for comparisons.