Table 3B.
PTI 72 h EC50 values (µM)
| Cell Line | Trimer44 (5a) EC50 | Trimer44NMe (5b) EC50 | Lys-Spm (6) EC50 |
|---|---|---|---|
| HPNE | ND | ND | ND |
| L3.6pl | 1.5 ± 0.1 | 3.2 ± 0.1 | 2.7 ± 0.2 |
| Panc-1 | 2.9 ± 0.15 | 3.11 ± 0.18 | 2.06 ± 0.14 |
| Su86.86 | 0.82 ± 0.03 | 1.96 ± 0.08 | 1.08 ± 0.01 |
| BxPC-3 | 0.34 ± 0.02 | 1.03 ± 0.09 | 0.25 ± 0.02 |
| AsPC-1 | ND | ND | ND |
| Capan 1 | ND | ND | ND |
| PanO2 | 0.61 ± 0.01 | 0.90 ± 0.07 | 6.00 ± 0.57 |
| CHOK1 | 0.34 ± 0.02 | 0.90 ± 0.07 | 1.37 ± 0.05 |
| CHOMG | ND | ND | ND |
aAll EC50 values were determined after 72 h incubation and represent the concentration of PTI (in µM) needed to provide a viability value halfway between the DFMO-only and the DFMO+Spd (1 µM) viability values. For example, when the DFMO-only control (added at its IC50 value) gave 50% viability and the DFMO+Spd control gave 100% viability, the EC50 value is the concentration of PTI needed to attain 75% viability in the presence of the IC50 dose of DFMO and the rescuing dose of Spd (1 µM) (Note: see Figure 3 for an illustration). Importantly, all EC50 values were well below the IC10 values noted in Table 3A.