Skip to main content
. 2016 Jun 1;6(6):1253–1270.

Figure 1.

Figure 1

CASZ1 is highly expressed in EOC tissues and EOC cell lines. (A, B) CASZ1 mRNA expression levels were evaluated in clinical EOC specimens using the (A) Affymetrix GeneChip HG-U133_Plus_2 (GSE44104), which included 3 normal ovarian tissues, 12 clear cell, 11 endometrioid, 28 serous, and 9 mucinous ovarian cancer tissues, and (B) 2 GEO databases (GSE6008 and GSE18520). CASZ1 expression levels in the experimental groups and the normal group were compared using Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.001. (C) CASZ1a and CASZ1b mRNA expression levels in human EOC cell lines were determined using qRT-PCR. CASZ1a and CASZ1b expression was normalized to β-actin expression and to CASZ1 expression in IOSE398 cells (an immortalized human ovarian surface epithelial cell line). The qRT-PCR data presented represent 3 independent experiments. (D) CASZ1a and CASZ1b protein levels in human EOC cell lines were determined using immunoblotting assays. β-actin was used as the internal control. (E) CASZ1a and CASZ1b expression in a normal ovarian tissue and tumor tissues derived from 28 EOC patients was assessed using qRT-PCR. The relative level of CASZ1 expression was normalized to β-actin levels and to CASZ1 levels in the normal tissue samples. The error bars represent the SEM.