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. 2016 Jun 1;6(6):1287–1301.

Figure 5.

Figure 5

Effect of HDAC inhibitors (sodium butyrate and TSA) on E-cadherin and various biomarkers of epigenetic regulators in A549 and H460 cells. (A) Treatment of cells with HDAC inhibitors restored the levels of E-cadherin and acetylated histone H3 (Lys 9) while decreasing the levels of HDAC 1, HDAC2 in A549 and H460 cells. Cells were treated with sodium butyrate (0, 1, 10 mM) and TSA (0. 0.1 and 1 µM) in culture for 24 h. Whole cell lysates or nuclear lysates were prepared and used for western blot analyses. Histone H3 antibody was used as a loading control. (B) Treatment with HDAC inhibitors decreased HDAC activity in cells. Cells were treated as detailed above, and nuclear lysates were used to measure the activity using HDAC Activity Assay Kit following the manufacture’s protocol. Significant difference vs. control group, *P<0.01, ¶P<0.05. (C) HDAC inhibitor treatment enhanced the levels of HAT activity in both A549 and H460 cell lines. Treatment protocols were identical to those detailed in (A and B). Significant increase vs non-treated control cells, **P<0.001; *P<0.01; ¶P<0.05.