Figure 1.

Effect of 30 mM troglitazone treatment for 72 h in KFs on Col1 and Egr1 expression. A. qRT-PCR analysis reveals levels of Col1 and Egr1 mRNA were reduced by approximately 50% when treated with troglitazone (*P = 0.018 and *P = 0.026). B. Western blot analysis indicated levels of Egr1 protein after normalization to β-actin were reduced when treated with troglitazone and quantified with Image J software. C. ELISA analysis indicated a reduced Col1 supernatant protein level in the troglitazone-treated KFs (*P = 0.031).